random hexanucleotide primer Search Results


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Promega random hexanucleotide primer
Random Hexanucleotide Primer, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Qiagen hexanucleotide random primers
Hexanucleotide Random Primers, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Carl Roth GmbH random hexamer primer mix
Random Hexamer Primer Mix, supplied by Carl Roth GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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InterLabService reverta kit containing m-mlv reverse transcriptase, random hexanucleotide primers, and total rna
Reverta Kit Containing M Mlv Reverse Transcriptase, Random Hexanucleotide Primers, And Total Rna, supplied by InterLabService, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Promega oligo-dt- und hexanucleotide-random primers
Oligo Dt Und Hexanucleotide Random Primers, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Boehringer Mannheim labeled by primer extension from random hexanucleotides
Fig. 5. Gal4-VP16 directs the HAT activity of the SAGA complex to promoter-proximal nucleosomes. The experimental set-up for these ‘scanning ChIPS’ is similar to that used in Figure ​Figure4:4: the template was incubated in the absence (–) or presence (+) of Gal4-VP16, and competitor chromatin was added where indicated. Next, the reactions were incubated with SAGA and acetyl-CoA (or mock acetylated in the absence of HAT complex), MNase digested and immunoprecipitated with anti-acetylated H3 antibody. DNA was extracted from the bound and unbound fractions and slot-blotted. The membranes were hybridized successively with a series of short probes (between 250 and 300 bp) that scan the length of the template, generated by PCR and labeled by primer extension from random <t>hexanucleotides</t> (Boehringer Mannheim). (A) Diagram showing the localization of the different probes when the plasmid is digested with BglI. (B) Average values and standard deviation of normalized data from three repeats of the experiment. The background signal (–HAT) was subtracted from the values obtained in the presence of SAGA for each condition. The numbers under the graph show the ratio of proximal (average of +A and –A) versus distal (average of +C and –C) signal for each condition tested. ND, not determined. (C) The reconstituted array was pre-incubated with Gal4-VP16 and competitor chromatin was added. Then, the template was acetylated by SAGA in the presence of acetyl-CoA, reactions were digested with MNase (+) or mock-digested (–), and the immunoprecipitation and slot blot were carried out as described above.
Labeled By Primer Extension From Random Hexanucleotides, supplied by Boehringer Mannheim, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Promega random hexanucleotide primers prime-a-gene
Fig. 5. Gal4-VP16 directs the HAT activity of the SAGA complex to promoter-proximal nucleosomes. The experimental set-up for these ‘scanning ChIPS’ is similar to that used in Figure ​Figure4:4: the template was incubated in the absence (–) or presence (+) of Gal4-VP16, and competitor chromatin was added where indicated. Next, the reactions were incubated with SAGA and acetyl-CoA (or mock acetylated in the absence of HAT complex), MNase digested and immunoprecipitated with anti-acetylated H3 antibody. DNA was extracted from the bound and unbound fractions and slot-blotted. The membranes were hybridized successively with a series of short probes (between 250 and 300 bp) that scan the length of the template, generated by PCR and labeled by primer extension from random <t>hexanucleotides</t> (Boehringer Mannheim). (A) Diagram showing the localization of the different probes when the plasmid is digested with BglI. (B) Average values and standard deviation of normalized data from three repeats of the experiment. The background signal (–HAT) was subtracted from the values obtained in the presence of SAGA for each condition. The numbers under the graph show the ratio of proximal (average of +A and –A) versus distal (average of +C and –C) signal for each condition tested. ND, not determined. (C) The reconstituted array was pre-incubated with Gal4-VP16 and competitor chromatin was added. Then, the template was acetylated by SAGA in the presence of acetyl-CoA, reactions were digested with MNase (+) or mock-digested (–), and the immunoprecipitation and slot blot were carried out as described above.
Random Hexanucleotide Primers Prime A Gene, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Meridian Bioscience hexanucleotide random primers
Fig. 5. Gal4-VP16 directs the HAT activity of the SAGA complex to promoter-proximal nucleosomes. The experimental set-up for these ‘scanning ChIPS’ is similar to that used in Figure ​Figure4:4: the template was incubated in the absence (–) or presence (+) of Gal4-VP16, and competitor chromatin was added where indicated. Next, the reactions were incubated with SAGA and acetyl-CoA (or mock acetylated in the absence of HAT complex), MNase digested and immunoprecipitated with anti-acetylated H3 antibody. DNA was extracted from the bound and unbound fractions and slot-blotted. The membranes were hybridized successively with a series of short probes (between 250 and 300 bp) that scan the length of the template, generated by PCR and labeled by primer extension from random <t>hexanucleotides</t> (Boehringer Mannheim). (A) Diagram showing the localization of the different probes when the plasmid is digested with BglI. (B) Average values and standard deviation of normalized data from three repeats of the experiment. The background signal (–HAT) was subtracted from the values obtained in the presence of SAGA for each condition. The numbers under the graph show the ratio of proximal (average of +A and –A) versus distal (average of +C and –C) signal for each condition tested. ND, not determined. (C) The reconstituted array was pre-incubated with Gal4-VP16 and competitor chromatin was added. Then, the template was acetylated by SAGA in the presence of acetyl-CoA, reactions were digested with MNase (+) or mock-digested (–), and the immunoprecipitation and slot blot were carried out as described above.
Hexanucleotide Random Primers, supplied by Meridian Bioscience, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Boehringer Mannheim formamide and random primer hexanucleotides pd(n)6
Fig. 5. Gal4-VP16 directs the HAT activity of the SAGA complex to promoter-proximal nucleosomes. The experimental set-up for these ‘scanning ChIPS’ is similar to that used in Figure ​Figure4:4: the template was incubated in the absence (–) or presence (+) of Gal4-VP16, and competitor chromatin was added where indicated. Next, the reactions were incubated with SAGA and acetyl-CoA (or mock acetylated in the absence of HAT complex), MNase digested and immunoprecipitated with anti-acetylated H3 antibody. DNA was extracted from the bound and unbound fractions and slot-blotted. The membranes were hybridized successively with a series of short probes (between 250 and 300 bp) that scan the length of the template, generated by PCR and labeled by primer extension from random <t>hexanucleotides</t> (Boehringer Mannheim). (A) Diagram showing the localization of the different probes when the plasmid is digested with BglI. (B) Average values and standard deviation of normalized data from three repeats of the experiment. The background signal (–HAT) was subtracted from the values obtained in the presence of SAGA for each condition. The numbers under the graph show the ratio of proximal (average of +A and –A) versus distal (average of +C and –C) signal for each condition tested. ND, not determined. (C) The reconstituted array was pre-incubated with Gal4-VP16 and competitor chromatin was added. Then, the template was acetylated by SAGA in the presence of acetyl-CoA, reactions were digested with MNase (+) or mock-digested (–), and the immunoprecipitation and slot blot were carried out as described above.
Formamide And Random Primer Hexanucleotides Pd(N)6, supplied by Boehringer Mannheim, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Promega random hexanucleotide-primer kit
Fig. 5. Gal4-VP16 directs the HAT activity of the SAGA complex to promoter-proximal nucleosomes. The experimental set-up for these ‘scanning ChIPS’ is similar to that used in Figure ​Figure4:4: the template was incubated in the absence (–) or presence (+) of Gal4-VP16, and competitor chromatin was added where indicated. Next, the reactions were incubated with SAGA and acetyl-CoA (or mock acetylated in the absence of HAT complex), MNase digested and immunoprecipitated with anti-acetylated H3 antibody. DNA was extracted from the bound and unbound fractions and slot-blotted. The membranes were hybridized successively with a series of short probes (between 250 and 300 bp) that scan the length of the template, generated by PCR and labeled by primer extension from random <t>hexanucleotides</t> (Boehringer Mannheim). (A) Diagram showing the localization of the different probes when the plasmid is digested with BglI. (B) Average values and standard deviation of normalized data from three repeats of the experiment. The background signal (–HAT) was subtracted from the values obtained in the presence of SAGA for each condition. The numbers under the graph show the ratio of proximal (average of +A and –A) versus distal (average of +C and –C) signal for each condition tested. ND, not determined. (C) The reconstituted array was pre-incubated with Gal4-VP16 and competitor chromatin was added. Then, the template was acetylated by SAGA in the presence of acetyl-CoA, reactions were digested with MNase (+) or mock-digested (–), and the immunoprecipitation and slot blot were carried out as described above.
Random Hexanucleotide Primer Kit, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/random hexanucleotide-primer kit/product/Promega
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Sileks GmbH hexanucleotides 6-random random hexa primer d0300
Fig. 5. Gal4-VP16 directs the HAT activity of the SAGA complex to promoter-proximal nucleosomes. The experimental set-up for these ‘scanning ChIPS’ is similar to that used in Figure ​Figure4:4: the template was incubated in the absence (–) or presence (+) of Gal4-VP16, and competitor chromatin was added where indicated. Next, the reactions were incubated with SAGA and acetyl-CoA (or mock acetylated in the absence of HAT complex), MNase digested and immunoprecipitated with anti-acetylated H3 antibody. DNA was extracted from the bound and unbound fractions and slot-blotted. The membranes were hybridized successively with a series of short probes (between 250 and 300 bp) that scan the length of the template, generated by PCR and labeled by primer extension from random <t>hexanucleotides</t> (Boehringer Mannheim). (A) Diagram showing the localization of the different probes when the plasmid is digested with BglI. (B) Average values and standard deviation of normalized data from three repeats of the experiment. The background signal (–HAT) was subtracted from the values obtained in the presence of SAGA for each condition. The numbers under the graph show the ratio of proximal (average of +A and –A) versus distal (average of +C and –C) signal for each condition tested. ND, not determined. (C) The reconstituted array was pre-incubated with Gal4-VP16 and competitor chromatin was added. Then, the template was acetylated by SAGA in the presence of acetyl-CoA, reactions were digested with MNase (+) or mock-digested (–), and the immunoprecipitation and slot blot were carried out as described above.
Hexanucleotides 6 Random Random Hexa Primer D0300, supplied by Sileks GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Amersham Life Sciences Inc random sequence hexanucleotide primers dna labelling megaprime kit
Fig. 5. Gal4-VP16 directs the HAT activity of the SAGA complex to promoter-proximal nucleosomes. The experimental set-up for these ‘scanning ChIPS’ is similar to that used in Figure ​Figure4:4: the template was incubated in the absence (–) or presence (+) of Gal4-VP16, and competitor chromatin was added where indicated. Next, the reactions were incubated with SAGA and acetyl-CoA (or mock acetylated in the absence of HAT complex), MNase digested and immunoprecipitated with anti-acetylated H3 antibody. DNA was extracted from the bound and unbound fractions and slot-blotted. The membranes were hybridized successively with a series of short probes (between 250 and 300 bp) that scan the length of the template, generated by PCR and labeled by primer extension from random <t>hexanucleotides</t> (Boehringer Mannheim). (A) Diagram showing the localization of the different probes when the plasmid is digested with BglI. (B) Average values and standard deviation of normalized data from three repeats of the experiment. The background signal (–HAT) was subtracted from the values obtained in the presence of SAGA for each condition. The numbers under the graph show the ratio of proximal (average of +A and –A) versus distal (average of +C and –C) signal for each condition tested. ND, not determined. (C) The reconstituted array was pre-incubated with Gal4-VP16 and competitor chromatin was added. Then, the template was acetylated by SAGA in the presence of acetyl-CoA, reactions were digested with MNase (+) or mock-digested (–), and the immunoprecipitation and slot blot were carried out as described above.
Random Sequence Hexanucleotide Primers Dna Labelling Megaprime Kit, supplied by Amersham Life Sciences Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Fig. 5. Gal4-VP16 directs the HAT activity of the SAGA complex to promoter-proximal nucleosomes. The experimental set-up for these ‘scanning ChIPS’ is similar to that used in Figure ​Figure4:4: the template was incubated in the absence (–) or presence (+) of Gal4-VP16, and competitor chromatin was added where indicated. Next, the reactions were incubated with SAGA and acetyl-CoA (or mock acetylated in the absence of HAT complex), MNase digested and immunoprecipitated with anti-acetylated H3 antibody. DNA was extracted from the bound and unbound fractions and slot-blotted. The membranes were hybridized successively with a series of short probes (between 250 and 300 bp) that scan the length of the template, generated by PCR and labeled by primer extension from random hexanucleotides (Boehringer Mannheim). (A) Diagram showing the localization of the different probes when the plasmid is digested with BglI. (B) Average values and standard deviation of normalized data from three repeats of the experiment. The background signal (–HAT) was subtracted from the values obtained in the presence of SAGA for each condition. The numbers under the graph show the ratio of proximal (average of +A and –A) versus distal (average of +C and –C) signal for each condition tested. ND, not determined. (C) The reconstituted array was pre-incubated with Gal4-VP16 and competitor chromatin was added. Then, the template was acetylated by SAGA in the presence of acetyl-CoA, reactions were digested with MNase (+) or mock-digested (–), and the immunoprecipitation and slot blot were carried out as described above.

Journal:

Article Title: Distribution of acetylated histones resulting from Gal4-VP16 recruitment of SAGA and NuA4 complexes

doi: 10.1093/emboj/19.11.2629

Figure Lengend Snippet: Fig. 5. Gal4-VP16 directs the HAT activity of the SAGA complex to promoter-proximal nucleosomes. The experimental set-up for these ‘scanning ChIPS’ is similar to that used in Figure ​Figure4:4: the template was incubated in the absence (–) or presence (+) of Gal4-VP16, and competitor chromatin was added where indicated. Next, the reactions were incubated with SAGA and acetyl-CoA (or mock acetylated in the absence of HAT complex), MNase digested and immunoprecipitated with anti-acetylated H3 antibody. DNA was extracted from the bound and unbound fractions and slot-blotted. The membranes were hybridized successively with a series of short probes (between 250 and 300 bp) that scan the length of the template, generated by PCR and labeled by primer extension from random hexanucleotides (Boehringer Mannheim). (A) Diagram showing the localization of the different probes when the plasmid is digested with BglI. (B) Average values and standard deviation of normalized data from three repeats of the experiment. The background signal (–HAT) was subtracted from the values obtained in the presence of SAGA for each condition. The numbers under the graph show the ratio of proximal (average of +A and –A) versus distal (average of +C and –C) signal for each condition tested. ND, not determined. (C) The reconstituted array was pre-incubated with Gal4-VP16 and competitor chromatin was added. Then, the template was acetylated by SAGA in the presence of acetyl-CoA, reactions were digested with MNase (+) or mock-digested (–), and the immunoprecipitation and slot blot were carried out as described above.

Article Snippet: The membranes were hybridized successively with a series of short probes (between 250 and 300 bp) that scan the length of the template, generated by PCR and labeled by primer extension from random hexanucleotides (Boehringer Mannheim). ( A ) Diagram showing the localization of the different probes when the plasmid is digested with Bgl I. ( B ) Average values and standard deviation of normalized data from three repeats of the experiment.

Techniques: Activity Assay, Incubation, Immunoprecipitation, Generated, Labeling, Plasmid Preparation, Standard Deviation, Dot Blot